2014 ASCB/IFCB Meeting abstracts
نویسندگان
چکیده
Since bioluminescence does not require excitation light, it is free from auto-fluorescence. Therefore, it has been used for quantitative analysis of gene expression and in vivo imaging. Furthermore, it is free from potential phototoxicity and is compatible with optogenetic tools. However application of bioluminescent imaging has been limited mainly by two drawbacks. Firstly, the light output from the bioluminescent protein or luciferase was much darker than fluorescent proteins (FPs), making it difficult to study fine structures such as cytoskeletal dynamics with luminescent imaging. Secondly, color variants of luciferase have been limited compared with FPs, precluding multicolor imaging.
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